Coding

Part:BBa_K1847001

Designed by: Anna Fomitcheva, Lisa Baumgartner   Group: iGEM15_ETH_Zurich   (2015-08-25)

lldr

LldR is a regulatory protein which represses ldhA and lldD, genes that encode for lactate dehydrogenates. This regulator can work as a repressor or activator. Looping the DNA seems to be the most likely strategy if it is compared to other operons1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 567
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

This protein has been characterized together with our synthetically designed promoters. You can find more information in: Part:BBa_K1847007,Part:BBa_K1847008,Part:BBa_K1847009.

Characterization and Improvement by LZU-CHINA iGEM 2022

Check the characterization and Improvement done by LZU-CHINA 2022 iGEM team in the experience page.


References

  1. J Bacteriol. 2008 Apr; 190(8): 2997–3005.


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